Isolation and characterization of keratin-like proteins from cultured cells with fibroblastic morphology.
نویسندگان
چکیده
منابع مشابه
Isolation and characterization of keratin-like proteins from cultured cells with fibroblastic morphology
Intermediate filaments (IF) isolated from a variety of cultured cells, conventionally described as fibroblasts, are composed predominantely of proteins of molecular weights of 54,000 and/or 55,000. Less than 15% of the protein found in native IF preparations from these cells is composed of three to four polypeptides of molecular weights 60,000-70,000. We have investigated some biochemical and i...
متن کاملADHESION AND MORPHOLOGY OF FIBROBLASTIC CELLS CULTURED ON DIAMOND-LIKE CARBON (DLC)-COATED AND UNCOATED Ti-13Nb-13Zr
Diamond-like carbon (DLC) coatings were deposited on Ti alloy (Ti-13Nb-13Zr) substrates using the plasma immersion process. The plasma immersion process, unlike the conventional techniques, allows the deposition of DLC on three-dimensional workpieces with high adhesion. We quantitatively evaluated the adhesion of fibroblasts on DLCcoated and uncoated Ti alloy at 2 h. Comparisons between sample ...
متن کاملIsolation and characterization of lymphocyte-like cells from a lamprey.
Lymphocyte-like cells in the intestine of the sea lamprey, Petromyzon marinus, were isolated by flow cytometry under light-scatter conditions used for the purification of mouse intestinal lymphocytes. The purified lamprey cells were morphologically indistinguishable from mammalian lymphocytes. A cDNA library was prepared from the lamprey lymphocyte-like cells, and more than 8,000 randomly selec...
متن کاملClonal Growth in Vitro of Human Cells with Fibroblastic Morphology
A methodology has been described for reliable cultivation in vitro of dispersed fibroblastic cells obtained from normal human organs. The procedure has permitted establishment of stable cell lines from almost every sample taken, among which the following organs were represented: skin, spleen, amnion, lung, liver, bone marrow, brain, muscle, and heart. Equally good growth has been achieved with ...
متن کاملIsolation and characterization ofLactococcus garvieae from diseased rainbow trout (Oncorhynchus mykiss, Walbaum) cultured in Iran
A total of 200 moribund rainbow trout with clinical signs of a hyperacute haemorrhagic septicemia were collected from rainbow trout farms in Fars, Kohkiloyeh-Boyer Ahmad and Charmohal-Bakhtiari provinces in the south and southwest of Iran during summer 2002 to winter 2008 for detection of Lactococcus garvieae, the causative agent of lactococcosis. Fish kidney samples were cultured aseptically o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1984
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.98.4.1231